Figure 5.

AZ inhibits the acidification of vesicles containing IAV virions. A, The pH standard curve of LysoSensor Yellow/Blue DND‐160 assay in A549 cells. B, The increase of pH by AZ (1, 5, 10 μmol/L), Baf A1 (50 nmol/L) and NH₄Cl (10 mmol/L) in A549 cells with the LysoSensor Yellow/Blue DND‐160 assay. C, Schematic diagram of NPC1‐pHluorin2 expression vector. D, The representative image of NPC1‐pHluorin2 A549 cells infected with Dil‐labelled WSN virus for 1 h. Scale bar, 10 μm. E, The pH standard curve of NPC1‐pHluorin2 assay in A549 cells. F, The pH increase of NPC1 + vesicles by AZ (10 μmol/L), CQ (10 μmol/L) and NH₄Cl (10 mmol/L) in NPC1‐pHluorin2 A549 cells induced with doxycycline. G, The pH increase of NPC1 + vesicles containing Dil‐labelled WSN virus by AZ (10 μmol/L), CQ (10 μmol/L) and NH₄Cl (10 mmol/L) in NPC1‐pHluorin2 A549 cells induced with doxycycline. Solvent was treated as Ctrl. All results are representative of three replicate experiments. ns, no significant, *P < .05, **P < .01, ***P < .001, ****P < .0001