Skip to main content
. 2020 Dec 21;40(3):e106501. doi: 10.15252/embj.2020106501

Figure 2. Human IFITMs inhibit infection by SARS‐CoV‐2.

Figure 2

  1. Flow cytometry histograms showing stable expression of the indicated IFITMs in HEK293T cells.
  2. Flow cytometry plots of HEK293T cells transfected with ACE2‐GFP and infected with SARS‐CoV‐2 (MOI 0.5) for 24 h showing SARS‐CoV‐2 infection occurs in ACE2‐GFP‐positive cells.
  3. Stable HEK293T lines as in (A) were infected with SARS‐CoV‐2 (MOI 1) for 24 h and analyzed for percent infection by flow cytometry. Representative example plots are shown for each cell line.
  4. Graph depicts normalized infection percentage measurements from 2 to 3 independent experiments, each performed in triplicate, from cells infected as in (C). Bars represent averages with individual data points shown as circles. Error bars represent SD. # P < 0.05 compared to vector control by ANOVA followed by Tukey’s multiple comparisons test.
  5. The indicated cell lines were infected with influenza A virus (IAV) for 24 h, and percent infection was determined by flow cytometry. Graph depicts percent infection of triplicate samples. Bars represent averages with individual data points shown as circles. Error bars represent SD. # P < 0.05. NS, not significant, by ANOVA followed by Tukey’s multiple comparisons test.

Source data are available online for this figure.