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. 2020 Dec 21;40(3):e106501. doi: 10.15252/embj.2020106501

Figure 4. Mouse IFITMs inhibit SARS‐CoV‐2 infection.

Figure 4

HEK293T‐ACE2‐GFP stable cells were transiently transfected with mouse (m) IFITM plasmids or vector control for 24 h.
  1. Flow cytometry histograms showing expression of each of the IFITM constructs with the dotted line indicating gating that was used for examining percent infections in IFITM‐expressing cells.
  2. Transfected HEK293T‐ACE2‐GFP cells were infected with SARS‐CoV‐2 (MOI 1) for 24 h and analyzed for percent infection by flow cytometry. Representative example plots are shown for each transfection condition.
  3. Graph depicts normalized infection percentage measurements from 2 to 3 independent experiments, each performed in triplicate, from cells infected as in (B). Bars represent averages with individual data points shown as circles. Error bars represent SD. # P < 0.05 compared to vector control by ANOVA followed by Tukey’s multiple comparisons test.
  4. Confocal microscopy of HEK293T cells transfected with WT mIFITM3 or mIFITM3‐Y20A. Green, anti‐IFITM3; blue, DAPI. White arrows highlight plasma membrane localization of IFITM3‐Y20A. Scale bars represent 10 µm.

Source data are available online for this figure.