Fig. 1.

Image analysis workflow for nuclear morphology in DCIS tissues. a, Hematoxylin and eosin (H&E)-stained whole slide image (WSI) of a representative DCIS tissue. Within each tissue, all ducts were identified and numbered as either; normal (blue) or DCIS (black). Scale bar, 2 mm. b, For each individual duct, epithelium was manually selected, nuclei were segmented by automated-detection and nuclear features were extracted using QuPath. Segmented images of nuclei were compared to manually annotated images. Scale bar, 20 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)