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. 2020 Nov 22;19(12):e13275. doi: 10.1111/acel.13275

Table 1.

Primers designed for real‐time RT‐qPCR experiments with SYBR Green

Gene Forward primer (5′−3′) Reverse primer (5′−3′)
G6PD GGAGGGCGACGACGACGAAG TCGGGCAGAAGGCCATCCCG
G6pd CCGGAAACTGGCTGTGCGCT CCAGGTCACCCGATGCACCC
Ggt1 CTCGGTGACCCAAAGTTTGTC GCGTAGAACTCAGAGCTCATGTTG
Glrx1 CCAGTGCGATTCAAGATTATTTACA GCCTATGCAGTCTTTACCTATGAAGA
Glrx2 TGTGAACCAGATCCAAGAAACAAT GGAACAGTAAGAGCAGGATGTTTTT
Ho1 GAGGCTAAGACCGCCTTCCT TTGTGTTCCTCTGTCAGCATCAC
Idh1 CATGTACCAGAAAGGGCAAGAGA CCTCGGGACCAGGCAAA
Me1 TGACCAAGGGACGTGCAA GGGAGAGTGACTGGATCAAAAGG
Mpo CATGCCCACCGAATGACA GCTTCGTCTGTTGTTGCAGTGT
nNos CCGCCAAAACCTGCAAAG TGTGGAGACGCACGAAGATG
Nrf2 GCCTTGTACTTTGAAGACTGTATGCA AAGCGACTCATGGTCATCTACAAAT
Pgd AGAGGCTTGGCCCCACAT CCGGTTCCCACTTTTGCA
Prdx6 GACGCTAACAACATGCCTGTGA CAGTTTCTTGTCAGGGCCAAA
Sod2 GGCCAAGGGAGATGTTACAA GCTTGATAGCCTCCAGCAAC
Txnrd1 CAGCGAGGAGACCATAGAGG GCACATTGGTCTGCTCTTCA
Txnrd2 GCTTCTGGCAAGGAAGACAC CCCTCAGCAACATCTCCAAT
Ndufs1 AATGAAGGGCTGGAATATCTTGAC CATCACACCTTCTCTGGCTTTCT
Sdha GGTGTTGCTGTGTGGCTGAT CATATCGCAGAGATCTTCCATACAA
Uqcrc2 ACCCGTGGGATTGAAGCA CGCCATGTTTTCCCTTGTTG
Mtco1 GCTAGCCGCAGGCATTACTATAC GCGGGATCAAAGAAAGTTGTG
Mtatp6 CACTATGAGCTGGAGCCGTAATT GAAGTGGGCAAGTGAGCTTTTT
Pgc1α ACCCACAGGATCAGAACAAACC TCTTCGCTTTATTGCTCCATGA
Pgc1β CGGAAGAACTTCAGACGTGAGA CATGTCACTGGAGAGATTTCGAAT
Pprc1 AGAAGGAGCGTGCAATAGAAGAG TTCAGCTCCGACCGAGTCAT
Pparα CCCTGTTTGTGGCTGCTATAATT GCAACTTCTCAATGTAGCCTATGTTT
Pparγ CCCAATGGTTGCTGATTACAAA AATAATAAGGTGGAGATGCAGGTTCT
Nrf1 TTGGCGCAGCACCTTTG GGTCTTCCAGGATCATGCTCTT
Tfam TCCAGGAGGCAAAGGATGAT TCCTCAGGAGACAGATTTTTCCA
Tfb1m CCGAGGGCTTGGAATGTTATT CAGCCTTCCAGTGCTTTCG
Tfb2m GGTCGTCATTTAGTGTGCATACG CTGATTCCCCGTGCTTTGA
Alas1 AGGAAAGAGGCTGCTCAAAGC ATCCTCTCCATCGGTTTTCACA
Ucp2 GCCTCTGGAAAGGGACTTCTC ACCAGCTCAGCACAGTTGACA
Sirt1 CGGCTACCGAGGTCCATATACTT TTCGAGGATCGGTGCCAAT
Sirt3 CGGCTCTATACACAGAACATCGA GTGGGCTTCAACCAGCTTTG

Primers were designed using Primer Express 3.0 software and the mouse gene sequences available on the Ensembl genome database.