Figure 1.

Anthocyanin‐rich mulberry extract inhibits aging‐induced senescence and oxidative stress in aging rats. (a, b) SA‐β‐gal staining of the aortas. Quantification analysis of SA‐β‐gal expression. (c) p21, p16, and expression of β‐actin in the aortas were determined by Western blotting. (d) Representative images of MitoSOX staining for MitoROS production and (e) quantification of fluorescence intensity for superoxide levels in aortas. (f) Representative fluorescent images of ROS formation in aortas were shown using ROS probe DHE. (g) Quantification of fluorescence intensity for superoxide levels in aortas. (h) NADPH oxidase activity determined by lucigenin chemiluminescence assay. Values are presented as mean ± SEM (n = 6, *p < 0.05 vs. the young group; #p < 0.05 vs. the aging group)