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Mulberry extract improved eNOS activity in aging rats. (a, b) Phosphorylation of Ser1177 and Thr495 and expression of total eNOS and β‐actin in the aorta were determined by Western blotting. (c) Representative images of the immunofluorescence staining of phosphorylation of eNOS at Ser1177 (p‐eNOS) on aortic sections. (d) Quantification of phosphorylation of eNOS at Ser1177. Values are presented as mean ± SEM (n = 6, *p < 0.05 vs. the young group; #p < 0.05 vs. the aging group)
