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. 2020 Dec 3;19(12):e13279. doi: 10.1111/acel.13279

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© 2020 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Mulberry extract improved eNOS activity in aging rats. (a) NAD⁺ and NADH levels were measured in the aortas, and NAD⁺/NADH ratios were quantified. (b) Sirtuin activity in the aortas. (c) SIRT1 and expression of β‐actin in the aortas were determined by Western blotting. (d) Acetylated lysine and expression of β‐actin in the aortas were determined by Western blotting. (e) Immunoprecipitation using an antibody against eNOS to evaluate acetylation levels and its association with SIRT1. Values are presented as mean ± SEM (n = 6, *p < 0.05 vs. the young group; ^# p < 0.05 vs. the aging group)