Figure 1.

Baicalein inhibited HSV-1 viral replication in vitro. (A) Vero and HaCat cells were incubated with baicalein or acyclovir at different concentrations for 72 h, and the cell viability was measured by CCK-8 assay (n = 6). (B) and (C) Vero and HaCat cells were pretreated with different concentrations of baicalein or acyclovir and then infected with HSV-1/F (MOI = 1) or HSV-1/Blue (MOI = 1) at 37 °C for 2 h. After infection, the cells were incubated with baicalein or acyclovir for an additional 24 h, and virus titers were determined by PFU assay (n = 3). (D) CC₅₀ and IC₅₀ of baicalein were calculated by linear regression analysis of the viral inhibition curves. SI was defined as the ratio of CC₅₀ to EC₅₀ (CC₅₀/EC₅₀). Data are presented as mean ± SD.