Fig. 1.

Induction of UGT1A1 by OCA in neonatal hUGT1 mice. The 10-day-old neonatal hUGT1 mice were treated with 50 mg/kg of OCA (divided in two doses of 25 mg/kg) or vehicle by oral administration for 5 consecutive days. (A) After the treatment, TSB levels were measured. (B) Liver and SI were used for gene and protein analysis. UGT1A1 gene expression was determined by RT-qPCR and expressed as fold induction. (C) Western blots were performed from liver and SI tissue to examine UGT1A1 expression. The bands have been cropped, and the full-length blots are in Supplemental Material. Values are means ± S.D. (n ≥ 3). Statistically significant differences between vehicle (Veh) and OCA are indicated by asterisks (Student’s t test: **P < 0.01). RT-qPCR, reverse-transcription quantitative polymerase chain reaction.