Fig. 2.

Oral OCA effect in liver of hUGT1 mice. Litters were bred to produce hUGT1 mice, and 10-day-old neonatal mice were treated with OCA or vehicle on day 10, followed by tissue preparation after treatment. (A) RNA was isolated from liver and used to conduct RT-qPCR for Cyp2b10, Shp, and Cyp7a1 genes.Supplemental Material (B) Total RNA was prepared from liver, and RT-qPCR was performed to measure expression of Cyp7a1, Cyp8b1, Cyp27a1, and Cyp7b1. (C) Western blots were performed using antibodies toward mouse CYP2B10 and mouse CYP7A1. The bands have been cropped, and the full-length blots are in Supplemental Material. Values are means ± S.D. (n ≥ 3). Statistically significant differences between vehicle (Veh) and OCA are indicated by asterisks (Student’s t test: *P < 0.05, ***P < 0.001). RT-qPCR, reverse-transcription quantitative polymerase chain reaction.