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. 2021 Jan;49(1):12–19. doi: 10.1124/dmd.120.000240

TABLE 1.

Primer sequences used for reverse-transcription quantitative polymerase chain reaction (RT-qPCR)

Primer Forward Reverse
Human UGT1A1 5′-CCT​TGC​CTC​ATC​AGA​ATT​CCT​TC-3′ 5′-ATT​GAT​CCC​AAA​GAG​AAA​ACC​AC-3′
Mouse Cyp2b10 5′-AAA​GTC​CCG​TGG​CAA​CTT​CC-3′ 5′-CAT​CCC​AAA​GTC​TCT​CAT​GG-3′
Mouse Nqo1 5′-TTT​AGG​GTC​GTC​TTG​GCA​AC-3′ 5′-GTC​TTC​TCT​GAA​TGG​GCC​AG-3′
Mouse Cyp1a1 5′-TGC​CCT​TCA​TTG​GTC​ACA​TG-3′ 5′-CAC​GTC​CCC​ATA​CTG​CTG​ACT-3′
Mouse Cyp4a10 5′-CAC​ACC​CTG​ATC​ACC​AAC​AG-3′ 5′-TCC​TTG​ATG​CAC​ATT​GTG​GT-3′
Mouse Cyp3a11 5′-ACA​AAC​AAG​CAG​GGA​TGG​AC-3′ 5′-CCC​ATA​TCG​GTA​GAG​GAG​CA-3′
Mouse Cyclophiline 5′-CAG​ACG​CCA​CTG​TCG​CTT-3′ 5′-TGT​CTT​TGG​AAC​TTT​GTC​TGC-3′
Mouse Shp 5′-CAC​GAT​CCT​CTT​CAA​CCC​AG-3′ 5′-AAG​ACT​TCA​CAC​AGT​GCC​CA-3′
Mouse Fgf15 5′-CAG​TCT​TCC​TCC​GAG​TAG​CG-3′ 5′-TGA​AGA​CGA​TTG​CCA​TCA​AG-3′
Mouse Cyp7a1 5′-GGG​AAT​GCC​ATT​TAC​TTG​GA-3′ 5′-GTC​CGG​ATA​TTC​AAG​GAT​GC-3′
Mouse Cyp8b1 5′-GAT​AGG​GGA​AGA​GAG​CCA​CC-3′ 5′-TCC​TCA​GGG​TGG​TAC​AGG​AG-3′
Mouse Cyp27a1 5′-GGG​CAC​TAG​CCA​GAT​TCA​CA-3′ 5′-CTA​TGT​GCT​GCA​CTT​GCC​C-3′
Mouse Cyp7b1 5′-TTA​TCA​AGG​GTG​GTT​CAC​GA-3′ 5′-TCC​TAG​GCC​TTC​TCT​TTG​CC-3′
Mouse Akp3 5′-CTG​GAG​CCC​TAC​ACC​GAC​T-3′ 5′-AGG​CTT​CTG​GCG​CTG​TTA​T-3′
Mouse Sis 5′-ACC​CCT​AGT​CCT​GGA​AGG​TG-3′ 5′-CAC​ATT​TTG​CCT​TTG​TTG​GAT​GC-3′
Mouse Krt20 5′-CCT​GCG​AAT​TGA​CAA​TGC​TA-3′ 5′-CCT​TGG​AGA​TCA​GCT​TCC​AC-3′
Mouse Glb1 5′-CAC​TGC​TGC​AAC​TGC​TGG-3′ 5′-ATG​TAT​CGG​AAT​GGC​TGT​CC-3′
Mouse Lrp2 5′-CCA​GGA​TTC​TGG​TGA​TGA​GG-3′ 5′-CGG​GAA​CTC​CAT​CAC​AAA​CT-3′
Mouse Nox4 5′-TCT​GGA​AAA​CCT​TCC​TGC​TG-3″ 5′-CCG​GCA​CAT​AGG​TAA​AAG​GA-3′

Glb1, galactosidase β1; Lrp2, low-density lipoprotein-related protein 2.