TABLE 1.
Measurements of contraction amplitude, contraction frequency, end diastolic diameter, and intraluminal flow in isolated rat mesenteric lymph vessels in response to three different KCOs
Exposure of LVs to either increasing quarter-log conc. of cromakalim (0.01–3 μmol/l), half-log conc. of minoxidil sulfate, or half-log conc. of diazoxide (0.01–100 μmol/l) progressively increased EDD concurrent with decreases in contraction frequency, amplitude, and calculated flow (*P < 0.05; **P < 0.01; ***P < 0.001;****P < 0.0001, mean ± S.D., n = 6).
| KCO | Conc. (µmol/l) | Amplitude (% Baseline) | Frequency (% Baseline) | End Diastolic Diameter (% Baseline) | Flow (% Baseline) |
|---|---|---|---|---|---|
| Cromakalim | 0.01 | 98.2 ± 5.4 | 102.1 ± 5.6 | 99.3 ± 0.9 | 99.5 ± 2.0 |
| 0.0178 | 100.6 ± 5.6 | 96.4 ± 8.1 | 99.2 ± 1.3 | 95.5 ± 4.6 | |
| 0.03 | 98.5 ± 5.7 | 96.4 ± 9.8 | 98.9 ± 1.3 | 93.7 ± 9.2 | |
| 0.056 | 98.4 ± 6.2 | 97.5 ± 12.2 | 98.2 ± 1.6 | 93.8 ± 9.4 | |
| 0.1 | 98.0 ± 9.4 | 86.9 ± 16.0 | 98.7 ± 1.4 | 83.2 ± 11.6 | |
| 0.178 | 100.1 ± 16.2 | 58.8 ± 28.0**** | 100.4 ± 2.3 | 57.7 ± 26.7**** | |
| 0.3 | 68.1 ± 53.3 | 39.4 ± 32.1**** | 102.3 ± 3.4 | 40.2 ± 32.6**** | |
| 0.56 | 81.7 ± 41.2 | 27.4 ± 19.7**** | 103.2 ± 3.1 | 28.3 ± 20.9**** | |
| 1 | 33.2 ± 52.9*** | 4.4 ± 7.2**** | 105.3 ± 3.0*** | 4.5 ± 7.1**** | |
| 1.78 | 0.0 ± 0.0**** | 0.0 ± 0.0**** | 106.4 ± 3.5**** | 0.0 ± 0.0**** | |
| 3 | 0.0 ± 0.0**** | 0.0 ± 0.0**** | 106.9 ± 3.7**** | 0.0 ± 0.0**** | |
| Minoxidil sulfate | 0.01 | 100.0 ± 2.3 | 96.2 ± 7.2 | 100.2 ± 0.5 | 96.5 ± 7.5 |
| 0.03 | 95.7 ± 11.6 | 90.9 ± 19.0 | 100.0 ± 1.8 | 86.9 ± 18.2 | |
| 0.1 | 96.0 ± 14.7 | 84.3 ± 23.2 | 100.3 ± 1.6 | 80.7 ± 22.4* | |
| 0.3 | 95.8 ± 19.7 | 56.9 ± 24.6**** | 101.6 ± 1.5 | 55.6 ± 26.2**** | |
| 1 | 56.1 ± 49.1** | 18.6 ± 21.8**** | 102.3 ± 1.4 | 17.9 ± 21.5**** | |
| 3 | 49.5 ± 54.4** | 4.0 ± 4.6**** | 103.2 ± 1.9** | 4.1 ± 4.6**** | |
| 10 | 0.0 ± 0.0**** | 0.0 ± 0.0**** | 103.7 ± 1.7** | 0.0 ± 0.0**** | |
| 30 | 0.0 ± 0.0**** | 0.0 ± 0.0**** | 103.9 ± 1.9*** | 0.0 ± 0.0**** | |
| 100 | 0.0 ± 0.0**** | 0.0 ± 0.0**** | 104.3 ± 1.8*** | 0.0 ± 0.0**** | |
| Diazoxide | 0.01 | 99.1 ± 6.5 | 99.4 ± 9.0 | 99.8 ± 0.9 | 98.4 ± 5.3 |
| 0.03 | 102.4 ± 10.9 | 95.0 ± 11.5 | 99.9 ± 1.2 | 96.3 ± 6.2 | |
| 0.1 | 101.7 ± 10.8 | 96.6 ± 10.5 | 99.6 ± 0.8 | 97.1 ± 5.8 | |
| 0.3 | 101.2 ± 11.6 | 93.8 ± 14.3 | 99.6 ± 0.7 | 93.8 ± 11.1 | |
| 1 | 100.0 ± 12.2 | 89.7 ± 8.3 | 100.3 ± 1.3 | 89.9 ± 7.3 | |
| 3 | 100.2 ± 10.2 | 82.6 ± 10.6* | 101.3 ± 2.2 | 83.9 ± 8.6* | |
| 10 | 101.3 ± 9.1 | 45.5 ± 19.5**** | 102.4 ± 6.5 | 47.1 ± 18.2**** | |
| 30 | 52.1 ± 57.5**** | 10.5 ± 17.4**** | 104.6 ± 7.4 | 10.2 ± 16.5**** | |
| 100 | 0.0 ± 0.0**** | 0.0 ± 0.0**** | 106.3 ± 7.1* | 0.0 ± 0.0**** |