TABLE 3.
Cycle thresholds for real-time PCR amplification using TaqMan primers specific for KATP channel subunits in whole-rat mesenteric LVs
Transcripts encoding all four known KATP channel subunits were detected, including the pore-forming Kir6.1 and Kir6.2 subunits and the regulatory SUR1 and SUR2 subunits. However, the SUR1 transcript was extremely sparse. GAPDH was the amplification standard. Data reported as mean ± S.D., n = 3.
| Channel Subunits | Cycle Thresholds |
|---|---|
| Kir6.1 | 25.9 ± 1.6 |
| Kir6.2 | 24.0 ± 1.6 |
| SUR1 | 31.6 ± 1.3 |
| SUR2 | 25.3 ± 1.7 |
| GAPDH | 25.7 ± 3.0 |