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. 2020 Jun 30;10(12):2299–2312. doi: 10.1016/j.apsb.2020.06.014

Figure 1.

Figure 1

BBR decreases PD-L1 expression in NSCLC cells. (A) Chemical structure of BBR. (B) IB analysis of PD-L1 expression in H460 cells treatment with the indicated natural compounds (10 μmol/L) for 24 h. (C) and (D) H460, H1975, H358 and HCC827 cells were treated with 10 μmol/L BBR for the indicated times (C), or treated with different concentrations of BBR for 24 h (D), PD-L1 and CD47 expression levels were detected by IB. (E) A549 or H1299 cells were pre-treated with 10 μmol/L BBR for 2 h, followed by IFN-γ (5 ng/mL) stimulation for 24 h, PD-L1 and IDO1 expression levels were detected by IB. (F) H157 cells were treated with BBR (5 and 10 μmol/L) for 24 h, the plasma membrane PD-L1 was detected by flow cytometry. Statistic of PD-L1 mean fluorescence intensity (MFI) is shown in right (n = 3). Data shown are mean value of three independent experiments±standard error of mean (SEM). ∗∗P < 0.01, ∗∗∗P < 0.001 compared with DMSO group.