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Evaluation of tumor growth and bone pain inhibition. (A) Relative tumor volumes and (B) Relative body weight of different treatment groups. (C) Spontaneous lifting time and (D) Number of flinches of the bone metastatic hindlimb. The arrows indicated the day of tail vein injection. Values were expressed as mean ± SD (n = 6, ∗∗P < 0.01, ∗∗∗P < 0.001). (E) H&E and Ki67 staining of the tumor tissues after various treatments. Scale bar: 200 μm.
