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. 2020 Dec 1;92(24):16149–16157. doi: 10.1021/acs.analchem.0c03858

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© 2020 American Chemical Society

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Analysis of triplex-labeled N-dime-GTDWLANK-GA-Ac (A) and BSA-yeast in the DIA mode (B). (A) From left to right, the peptide-coupled reporter ion of a single channel of the precursor ion of N-dime-GTDWLANK-GA-Ac (+2) and the peptide-coupled reporter ions in the DIA MS2 spectra at ratios of 1:2:5, 5:2:1, 1:10:20, and 20:10:1; (B) analysis of the BSA-yeast sample in the DIA mode. Log2-normalized protein ratio distribution of triplex-labeled LysC yeast peptides mixed at a ratio of 5:2:1 combined with LysC BSA peptides mixed at a ratio of 1:5:10. Expected values for log2-normalized mixing ratios are shown as dotted lines.