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Immunofluorescence staining of NEK7, the NLRP3 inflammasome, and α-smooth muscle actin (α-SMA) in arteries of patients with DLEAD. The yellow bar represents 100 µm, and the white bar represents 200 µm. Con, control group; DAPI, 4',6-diamidino-2-phenylindole; DLEAD, diabetic lower extremity arterial disease; n=4 at least in each group.
