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. 2020 Dec 3;5(49):31792–31800. doi: 10.1021/acsomega.0c04489

Figure 1.

Figure 1

Effects on cell viability of hot water extracts of herbs on CRC cell lines. HCT116, HT-29, SW620, or DLD-1 cells were seeded in 96-well plates at a density of 2000 cells/well and incubated overnight. Cells were then treated with hot water extracts of herbal tea with a concentration of 100, 500, and 1000 μg/mL for 48 h. After 48 h treatment, cell viability was measured by the MTS assay at 490 nm. The results were normalized to water control and shown in mean (% of control) ± SD of three sampled replicates. Dash lines indicate 100% viability. *, p < 0.01; **, p < 0.001; ***, and p < 0.0001 compares the 100 μg/mL of extracts to the swater control. #, p < 0.01; ##, p < 0.001; and ###, p < 0.0001 compares the 500 μg/mL of extracts to the water control. +, p < 0.01; ++, p < 0.001; and +++, p < 0.0001 compares the 1000 μg/mL of extracts to water control.