Figure 2.

MO extract inhibited cell growth in HCT116 cells. (A) HCT116 cells were seeded in 10 cm dish overnight and then treated with control (water) or MO for 48 h with the concentration ranging from 0 to 500 μg/mL for HCT116 cells. After 48 h treatment, the cellular morphology was observed by optical microscopy before cell counting by trypan blue exclusion staining. (B) MO-pre-treated-48 h cells were seeded in 6-well plates (250 cells/well) with MO-free medium and incubated for eight days for HCT116. Formed colonies were fixed, stained, and counted. All experiments were performed in three independent replicates. Significant difference indicated as ***p < 0.001, **p < 0.01, *p < 0.05 compared to 0 μg/mL control.