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. 2020 Dec 15;8(2):e001748. doi: 10.1136/jitc-2020-001748

Figure 2.

Figure 2

Detection of TCR-T cell cytotoxicity. (A) Cytotoxicity assays were performed by incubating TCR-T cells with T2 cells pulsed with vrt1 (10−6 to 10−13 M) at an E:T ratio of 1:1. (B) To verify specificity, LDH release was also measured for unpulsed and irrelevant peptide-pulsed T2 cells. (C) Cytotoxicity of LDH release was determined after incubating TCR-T cells with HLA*A-02:01- or HLA*A-02:01+ PLC/PRF/5 HCC cell lines at an E:T ratio of 1:1 or 3:1. The negative controls were HBsAg-negative HepG2 cells and TCR-A6-T cells. All assays were performed in triplicate. *p<0.05, **p<0.01, ***p<0.001. E:T, effector-to-target; HBsAg, HBV surface antigen; HBV hepatitis B virus; LDH, lactate dehydrogenase; TCR, T-cell receptor; TCR-T, TCR-engineered T cell.