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. 2020 Dec 15;48(12):0300060520978015. doi: 10.1177/0300060520978015

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — miR-708-5p negatively regulates the osteoblast differentiation of BMSCs. (a) Alizarin Red S staining (100× magnification) showing that mir-708-5p inhibited the deposition of osteogenic calcium 14 days after BMSC osteoblast induction. (b) Quantitative analysis of ALP activity in BMSCs. mir-708-5p inhibited the ALP activity of osteoblasts. (c, d) Quantitative PCR and western blot analyses showing the effect of miR-708-5p on the mRNA and protein expression of BMP2, BMP4, and Runx2 in osteoblasts. Data are the means ± SD of three independent experiments. *P < 0.05 and ** P < 0.01 versus control.

OSI, osteogenic induction group; CON, non-osteogenic induction group; BMSCs, bone marrow mesenchymal stem cells; ALP, alkaline phosphatase; BMP, bone morphogenetic protein; Runx2, Runt-related transcription factor 2.