The effects of topical application of 0.05% MZR in PBS and Restasis on CD4+ T-cell-based inflammation in the conjunctiva of mice under DS. (A) Representative images of CD4+ T-cell staining (scale bar: 500 µm). (B) Statistical analysis of the number of CD4+ T cells in conjunctiva per millimeter. Statistical; analysis of gene expression and protein levels of IFN-γ (C, D), IL-13 (E, F), and IL-17A (G, H) in the conjunctiva. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 5 for each group).