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. 2020 Dec 15;9(13):22. doi: 10.1167/tvst.9.13.22

Figure 4.

Figure 4.

The effects of topical application of 0.05% MZR in PBS and Restasis on apoptosis in the ocular surface of mice under DS. (A) Representative images of AC-caspase-8 immunofluorescence staining in the conjunctiva (scale bar: 300 µm). (B) Representative images of protein level of AC-caspase-8 in the conjunctiva. (C) Statistical analysis of the protein level of AC-caspase-8 in the conjunctiva. (D, F) Representative images of TUNEL staining in the cornea and the conjunctiva (scale bar: 50 µm). (E, G) Statistical analysis of the number of TUNEL-positive cells in the cornea and the conjunctiva. Data are shown as mean ± SD. *P < 0.05, **P <0.01, ***P < 0.001 (n = 5 for each group).