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Histological examinations of day-19 chicken embryonic gonads for expression of SOX9 and DMRT1 (A) and SOX9 and SYCP3 (B). Serial slides of cryosections were subjected to H&E staining and immunofluorescence. All fluorescence images in each row were taken from the same slide. Scale bars indicate 100 µm, and dots indicate borders of cortex (C). Abbreviation: H&E, hematoxylin and eosin.
