Table 1.
Groups of DEGs in day-19 chicken embryonic gonads
| Representing Gene | Response to Feminization | Number of DEGs | Messenger RNA Expression | Link to chrZ | ||||
|---|---|---|---|---|---|---|---|---|
| FS = 0 ZZ-testes |
FS = 1 Weak FemZZ |
FS = 2 Strong FemZZ |
FS = 3 EE2-expopsed ZW-ovary |
FS = 3 Non-exposed ZW-ovary |
||||
| SEMA3C | Induced (high response) |
63 | - | ++/+++ | +++ | +++ | +++ | 3% |
| CYP19A1 | Induced (modest response) |
46 | - | -/+ | + | ++ | +++ | 4% |
| ZP3 | Induced (low response) |
5 | - | -- | - | +++ | +++ | 0% |
| CTNNB1/AMH | Suppressed (high response) |
146 | +++ | + | -/+ | - | - | 5% |
| SKIV2L2/DMRT1 | Suppressed (low response) |
21 | +++ | ++/+++ | ++/+++ | - | - | 90% |
| ACVR2B | Suppressed (low response) |
4 | +++ | ++/+++ | ++/+++ | + | - | 50% |
| CBL | FemZZ-specific induction |
8 | - | ++/+++ | ++/+++ | ++ | + | 0% |
| DMRTB1 | FemZZ-specific induction |
9 | -/+ | ++/+++ | ++/+++ | +/++ | -/+ | 0% |
| DAZL | FemZZ-specific induction |
21 | -/+ | ++/+++ | ++/+++ | -/+ | -/+ | 5% |
| IL1R1 | FemZZ-specific suppression | 19 | +++ | + | -/+ | -/+ | +++ | 0% |
| REPS2 | FemZZ-specific suppression | 9 | +++ | + | -/+ | ++/+++ | +++ | 0% |
Symbols indicate relative expression (-, no expression; -/+ less than 20%; +, 20-50%; ++, 51-80%; and +++, 81-100% expression).
Abbreviations: chrZ, chromosome Z; DEGs, differentially expressed genes; EE2, ethynylestradiol; FemZZ, feminized genetic male testes; FS, feminization score; ZZ-tested, genetic male testes; ZW-ovary, genetic female ovary.