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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Arthritis Rheumatol. 2020 Aug 14;72(9):1505–1513. doi: 10.1002/art.41288

Figure 3. Reduced T cell cytokine production in response to collagen due to blocking or absence of CD6.

Figure 3.

Th1//Th17/Th9 recall assays in UMCD6 treatment studies show that splenocytes from the IgG treated CIA group, but not the UMCD6 treated CIA mice, showed reactivity to collagen in vitro as measured by secretion of IFN-γ, IL-17 and IL-9. Controls include no antigen and a non-relevant antigen (OVA 323–339 peptide) (upper panel). We also performed collagen-specific recall assays using splenocytes collected from CD6 KO or Wt mice after CIA induction. These assays demonstrated significantly reduced collagen-specific Th17 and Th9 but not Th1 responses (lower panel). The ELISA assays have sensitivities in the picogram range for each of the cytokines tested. Data are mean ± SEM (*p<0.05).