Figure 2.

Detailed immunological characterisation of CD39⁺ CD8 tumor-infiltrating lymphocytes (TILs) from the ovary according to programmed cell death protein 1 (PD-1) expression level. (A) CD39⁺ CD8 TILs were divided into three subpopulations based on PD-1 expression level. The left image is a representative flow cytometry contour plot, with the gray box indicating the subpopulation without PD-1 expression (PD-1^neg), the blue box intermediate PD-1 expression (PD-1^int) and the red box high PD-1 expression (PD-1^high). The bar on the right shows the average percentages of the PD-1^neg, PD-1^int and PD-1^high subpopulations among all patients. (B) Percentages of cytotoxic T-lymphocyte-associated protein 4 (CTLA-4)⁺ and Tim-3⁺ cells among CD39⁺ CD8 TILs according to the differential expression of PD-1. Contour plots on the left are representative plots. (C–F) Frequencies of Eomes^high T-bet^low (C), Tcf-1⁺ (D), CD103⁺ (E) and CD38⁺ HLA-DR⁺ (F) cells among subpopulations according to differential expression of PD-1. Left panel shows representative flow cytometry plots for each. (G) Correlation between the frequencies of Tcf-1⁺ cells and CD38⁺ HLA-DR⁺ cells among CD39⁺ CD8 TILs from the ovary. Graphs show the mean and SD. **P<0.01; ****p<0.0001.