Fig. 2. Bioinformatics studies indicate the correlation between HNF4A-AS1 and HNF4α.

(A) A schematic view of HNF4A/HNF4A-AS1 genomic structure and the construction principle of reporter vectors with HNF4A-AS1. Purple ellipse: HNF4A-P2 promoter; Purple rectangle: P2-exon1; Cyan arrows: The indicator of 5’ and 3` orientation. Blue ellipse: The proximal promoter of HNF4A-AS1; Blue rectangle: HNF4A-AS1 exons; Red arrow: coding sequence of HNF4A-AS1 proximal promoter. Green arrow: The reverse complementary sequence of red arrow; (B) The ChIP-seq data showing the binding of HNF4α to the promoter of HNF4A-AS1 and P1-HNF4A in liver and HepG2 cells.