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. 2020 Nov 30;9:e61481. doi: 10.7554/eLife.61481

Figure 4. Kinesin-binding protein (KBP) binds kinesin MDs via conserved motifs in the α-solenoid edge loops and α-helices at the concave face.

(a) Pseudo-atomic model of the KBP–KIF15_MD6S complex (ribbon representation) displayed in cryo-electron microscopy density, using the same viewpoint as Figure 2a, but with the KIF15_MD6S now coloured by subdomain as in Figure 3. The KIF15_MD6S Switch 1/2 subdomain (Kin S1/2 sd) is coloured sienna, and the P-loop subdomain (Kin-PLsd) is coloured light pink. The KIF15_MD6S tubulin-binding subdomain (TBsd) is coloured magenta. The KBP subdomains are coloured as labelled. The nine helix pairs of KBP are labelled. Semi-transparent density is coloured regionally as per the fitted model and additional density for the linker loop is shown in semi-transparent black. (b) The same as panel a, but rotated 45° and 15° respectively around the axes indicated. (c) Zoomed view of the region indicated in panel a, with density removed and selected KIF15_MD6S and KBP secondary structure elements labelled. (d) Zoomed view of the region indicated in panel b, with density removed and selected KIF15_MD6S and KBP secondary structure elements labelled.

Figure 4.

Figure 4—figure supplement 1. Additional kinesin-binding protein (KBP) α-solenoid edge loops proximal to KIF15_MD6S.

Figure 4—figure supplement 1.

Colouring and representation as in Figure 4. (a) A view showing α-helical pairs αHP1, αHP2, αHP3, αHP8, and αHP9 of KBP and the KIF15_MD6S coloured by subdomain as labelled. (b) Left zoomed region in panel a, with density removed, showing KBP L1, L3, and L5 and proximal kinesin elements KL9, KL11, and Kα6. ADP is coloured in light orchid. (c) Right zoomed region in panel a, with density removed, showing KBP L16 and L18 and proximal KIF15_MD6S elements KNT (kinesin N-terminus), KNL (kinesin neck-linker), and KL12.