Author response image 1. KIF15 localizes on microtubules in the peroxisome motility assay.
(a, b) Representative images of COS-7 cells expressing KIF15_MDC–HA–FRB and PEX–mRFP–FKBP treated with rapalog for three hours and co-stained for (a) microtubules (rabbit anti-α-tubulin, 1:1000, Abcam) or (b) F-actin (Phalloidin Alexa647, 1:40, Life technologies). Zooms of the boxed regions are shown on the right. Scale bars, 10 µm.