Table 2. Kinesin-binding protein (KBP) mutants used in this study.

The original and mutated amino acid (top) and nucleotide sequences (bottom) are shown for each construct.

Construct	Original sequence	Mutated to
L1	EKEPYK gagaaggaaccatacaag	AAAPAA gcagcagcaccagcagca
L3	TEE acggaggag	AGG gcaggagga
L5	REE agagaagaa	AGA gcaggagca
L10	KISATEDTPEAEGEVPEL aagatctcagccacagaagacactcctgaagctgaaggagaagtgccagagctt	AGAGAGAGPAGAGAGPGG gcaggagcaggagcaggagcaggaccagcaggagcaggagcaggaccaggagga
L12	DGY gatggttat	GGA ggcggcgcg
L14	DLNPQY gacctgaatccacagtat	AAGPAA gcagcaggaccagcagca
L16	NKVFPEHIGEDVL aataaagtattccctgagcatataggggaagatgttctt	AAGAPAGAGAGAA gcagcaggagcaccagcaggagcaggagcaggagcagca
L18	EKHPE gaaaagcatcctgag	AAGPG gcagcaggaccagga
αHP4a	YLAQ tacctagctcaa	ALAA gcactagctgca
αHP4b	Q cag	A gca
αHP5a	TLSQ accttgtcacag	ALSA gcgttgtcagcg