Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Neurospora crassa) | prd-2 | FungiDB | NCU01019 | |
| Gene (Neurospora crassa) | upf1prd-6 | FungiDB | NCU04242 | |
| Gene (Neurospora crassa) | ck-1a | FungiDB | NCU00685 | |
| Strain, strain background (Neurospora crassa) | Supplementary file 1 | This study; Fungal Genetics Stock Center (FGSC) | ||
| Antibody | Anti-V5 (mouse monoclonal) | ThermoFisher | Cat. # R960-25 | (1:3000) |
| Antibody | Anti-tubulin alpha (mouse monoclonal) | Fitzgerald | Cat. # 10R-T130a | (1:10,000) |
| Antibody | Anti-CKI (rabbit polyclonal) | Generous gift from Michael Brunner (University of Heidelberg) | (1:1000) | |
| Antibody | Anti-FLAG M2 magnetic beads (mouse monoclonal) | Sigma | Cat. # M8823 | 30 μl beads incubated with 10 mg total protein for UV-CLIP |
| Recombinant DNA reagent | c box-luc (plasmid-derived construct) | As described, PMID:25635104 | <500 bp of the frq promoter driving codon-optimized luciferase; targeted to the csr-1 locus for selection |
|
| Chemical compound, drug | D-quinic acid | Sigma | Cat. # 138622 | 1 M stock solution, pH adjusted to 5.8 with NaOH |
| Chemical compound, drug | Allele-In-One Mouse Tail Direct Lysis Buffer | Allele Biotechnology | Cat. # ABP-PP-MT01500 | 50 μl reagent mixed withNeurospora asexual spores for gDNA isolation |
| Chemical compound, drug | Thiolutin | Cayman Chemical | Cat. # 11350 | Stock solution prepared in DMSO |
| Software, algorithm | Custom R software | https://github.com/cmk35 | UTR length analyses from Figure 5—figure supplement 2 |