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. 2020 Dec 7;16(12):e1009128. doi: 10.1371/journal.ppat.1009128

Fig 1. SARS-CoV-2 spike protein inhibits ACE2 expression.

Fig 1

(A, B) Western blot analysis of SARS-CoV-2 spike protein expression in Huh7.5 and A549 cell lysates prepared after 48 h of mock- or SARS-CoV-2 virus infection, or transiently transfected with an empty vector or SARS-CoV-2 spike gene construct. (C, D) Western blot analysis of ACE2 and AT1 receptor expression in Huh7.5 and A549 cell lysates prepared after 48 h of mock- or SARS-CoV-2 virus infection, or transiently transfected with empty vector or SARS-CoV-2 spike gene construct. (E, F) Western blot analysis of ACE2 expression in Huh7.5 and A549 cell lysates prepared after 48 h of transfection of empty vector or SARS-CoV-2 spike S1 or S2 gene construct. Expression level of actin or tubulin in each lane from the same gel is shown as a total protein load for comparison.