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. 2020 Dec 7;16(12):e1009128. doi: 10.1371/journal.ppat.1009128

Fig 5. SARS-CoV-2 spike protein regulates ADAM-17 activity.

Fig 5

(A) ADAM-17 enzymatic activity was measured from crude extracts of Huh7.5 and A549 cells after transfection of SARS-CoV-2 spike gene constructs. (B) ADAM-17 enzymatic activity was measured from crude extracts of Huh7.5 and A549 cells after transfection of SARS-CoV-2 spike S1 or S2 gene construct or empty vector. The results are presented as mean ± standard deviation. ‘*’ represent statistical significance (p<0.05). (C, D) Western blot analysis of ACE2 expression in Huh7.5 and A549 cell lysates prepared after 48 h of transfection of empty vector or SARS-CoV-2 spike S1 gene construct in the presence or absence of ADAM-17 inhibitor. Expression level of actin in each lane from same gel is shown as a total protein loading control for comparison.