Fig 6. TAD disruptions tend to occur at insulator-like regions.

We analyzed HiCExplorer insulation scores calculated for 5 kb bins across the D. melanogaster and D. triauraria genomes. A) We found that intra-TAD breakpoints tend to occur at genomic regions that have increased insulation relative to other intra-TAD bins: Wilcoxon test p < 2.2e–16, but significantly less insulation compared to TAD boundaries: Wilcoxon test p = 1.2e–12. B) We also examined lineage-specific boundaries and found that the genomic regions orthologous to lineage-specific boundaries show increased insulation relative to all intra-TAD 5 kb bins: Wilcoxon test p < 2.2e–16. However, lineage-specific boundaries have significantly increased insulation compared to the orthologous region in the other species, supporting their classification as lineage-specific: Wilcoxon test p = 4e–8. Note that more negative scores indicate more insulation. Full plot for B in S9 Fig.