Fig 1. Loss of CAC1 or ASF1, but not RTT106, restores silencing at HMRae**.

A) Map of HMRae**. B) Mutants with defects in H4 K16ac promote Sir protein binding and restore silencing at HMRae**. 1, 2, 3, and 4 are Sir1p, Sir2p, Sir3p, and Sir4p, respectively. C and D) Patch Mating Assays. Cells with the indicated genotypes were grown on YPD at 30°C overnight, then were replica plated onto minimal medium (YM plate) with a MATa lawn (JRY2726) and were grown at 30° for two days prior to imaging. Only cells that were silenced at HMRae** mated and grew as diploids on the MATa plate. E) Flow Cytometry. Yeast with the indicated genotypes were grown logarithmically in YPD at 30°C prior to harvesting to assess cell cycle distribution by Flow Cytometry.