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. 2020 Dec 7;16(12):e1009226. doi: 10.1371/journal.pgen.1009226

Fig 3. Cac1p and Asf1p interact in a cell cycle- and RTT109-dependent manner in vivo.

Fig 3

(A-C) Interaction between Cac1p and Asf1p is RTT109-dependent. A) Confocal fluorescence lifetime images of GFP in small-budded live cells expressing the fluorescently-tagged proteins as indicated. B) The average lifetime of GFP in indicated strains. C) FRET efficiency of indicated strains. (D-F) Interaction between Cac1p and Asf1p is cell cycle-dependent. D) Confocal fluorescence lifetime images of GFP in unbudded and budded live cells expressing the fluorescently-tagged proteins as indicated. Left panels arrows = unbudded cells. E) The average lifetime of GFP in indicated strains. F) FRET efficiency of indicated strains. Error bars represent the standard deviation of ten FLIM measurements taken for each genotype. White scale bars are equivalent to 5μm. FLIM scale bar: 1 nanosecond, blue; 3 nanoseconds, red.