Skip to main content
. 2020 Dec 7;16(12):e1009226. doi: 10.1371/journal.pgen.1009226

Fig 5. Defects in factors associated with sister chromatid cohesion restore silencing at HMRae**.

Fig 5

A-B) Restoration of silencing at HMRae** in mms1Δ, mms22Δ (A), and rtt101Δ (B) mutants required SIR2. C) Loss of CTF4 restored silencing at HMRae**. For Patch Mating assays, cells with the indicated genotypes were analyzed as in Fig 1D) Negative synthetic interaction between ctf4Δ and cac1Δ mutants. Tetrads from diploid strain ade2-1/ADE2 cac1Δ/CAC1 CTF4/ctf4Δ were dissected onto YPD plates and incubated three days at 30°C prior to imaging.