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. 2020 Dec 7;16(12):e1009226. doi: 10.1371/journal.pgen.1009226

Fig 7. H4 K16 is hypoacetylated at HMRae** in cells lacking RTT101.

Fig 7

H4 K16ac levels at the e** silencer and a1 in MATα HMRae** sir2Δ strains with the indicated genotypes were monitored by ChIP. The efficiency of coprecipitation of DNA with H4 K16ac antibodies was determined relative to that with anti-H3 antibodies, and normalized to yeast lacking SIR2, which was set to 1. Data were calculated as 2[(H4 K16ac CT - H3 CT)WT - (H4 K16ac CT - H3 CT)genotype]. Mean ± STD, n = 4 or 5. Each mutant was hypoacetylated relative to WT at both e** and a1 (p = 0.08 for cac1Δ at e**; p = 0.007 for all other samples and loci; Wilcoxon Rank Sum Test).