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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Neuroscience. 2019 May 7;410:274–292. doi: 10.1016/j.neuroscience.2019.04.047

Fig. 8. Sex differences in Mapk1 expression and MAPK and pMAPK protein densities following oxycodone CPP.

Fig. 8.

A. In the medial hippocampus (MH), Mapk1 expression is significantly lower in Oxy-females (OF) compared to Oxy-males (OM). B. In CA1 (left), MAPK-ir is densest in the pyramidal cell layer (PCL) and stratum lacunosum-moleculare (SLM) but is also found throughout the other lamina (SO, nSR, dSR). In DG (right), MAPK-ir is dense in both the dorsal and ventral granule cell layers (dGCL, vGCL) and in the central hilus (cen). C. Representative light microscope photographs show distribution of pMAPK-ir in the CA1 region. D. Representative light microscope photographs show distribution of pMAPK-ir in the dentate gyrus. SF, Sal-female; SM, Sal-male. Arrows indicate examples of pMAPK-labeled cells. Bars = 25 μm. E & F. In CA1, the density of pMAPK-ir significantly decreased in the nSR and dSR of Oxy-females compared to Sal-females. Moreover, Oxy-females had significantly lower densities of pMAPK-ir in SO and nSR and a trend towards lower density of pMAK-ir in dSR and SLM compared to Oxy-males. G & H. In the dentate gyrus, Sal-females had significantly higher densities of pMAPK-ir the dGCL and central hilus compared to Sal-males. Sal-females had significantly higher densities of pMAPK-ir than Oxy-females in the vGCL and in the central hilus. Sal-males had a trend towards lower density of pMAPK-ir than Oxy-males in the central hilus. Oxy-females compared to Oxy-males had significantly lower densities of pMAPK-ir in the vGCL and central hilus. *p < 0.05, ap = 0.034, bp = 0.013, cp = 0.061, dp = 0.063, ep = 0.040; fp = 0.030, gp = 0.041; hp = 0.006.