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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Nat Methods. 2020 Aug 10;17(9):917–921. doi: 10.1038/s41592-020-0926-5

Extended Data Figure 7: AC-ER dynamically co-accumulates with F-actin during actin wave cycling.

Extended Data Figure 7:

Actin waves were imaged in HeLa cells co-expressing AC-ER and the F-actin marker LifeAct. Boxes mark regions of LifeAct accumulation at different timepoints. Graphs displaying normalized pixel intensity over time in the boxed regions are displayed as insets. This figure represents chosen timepoints from Supplementary Videos 8 and 9. The actin wave dynamics are much more easily visualized in the movies. Scale bar: 5 µm. These results were reproducible across 4 independent experiments.