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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Nat Methods. 2020 Aug 10;17(9):917–921. doi: 10.1038/s41592-020-0926-5

Extended Data Figure 10: Mitochondria- and ER-associated actin accumulates during ER-mediated mitochondrial fission.

Extended Data Figure 10:

A) AC-ER specifically accumulates at fission sites prior to ER-mediated fission. AC-ER accumulation is evident during ER-mediated mitochondrial fission. Hollow arrows mark the location of mitochondrial fission. Yellow lines in the left column mark the area used to generate the line scans. Scale bar: 1 µm. Example images of the areas used to determine the degree of MitoTracker overlap and coefficient of variance for mCherry/AC-ER and the resulting values are also shown. These results were reproducible across 19 independent experiments. B) AC-mito accumulates prior to ER-mediated fission. Same as A with cells expressing AC-mito instead of AC-ER.