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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Nat Methods. 2020 Aug 10;17(9):917–921. doi: 10.1038/s41592-020-0926-5

Extended Data Figure 3: AC-mito expression does not alter endogenous Fis1 localization to mitochondria.

Extended Data Figure 3:

Maximum projection of cells expressing AC-mito and non-transfected cells show similar levels of endogenous anti-Fis1 immunofluorescence. Note that the anti-Fis1 antigen is the Fis1 N-terminus, which is not present in the AC-mito protein, which contains only the C-terminus of Fis1. Scale bar: 20 µm. These results were reproducible across 2 independent experiments.