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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Nat Methods. 2020 Aug 10;17(9):917–921. doi: 10.1038/s41592-020-0926-5

Extended Data Figure 4: Alternate membrane-targeting and actin-binding motifs yield similar results.

Extended Data Figure 4:

Switching the actin nanobody motif with LifeAct (Fis1-LifeAct-GFP) yields similarly increased accumulation compared to control constructs as quantified by the coefficient of variance. Switching the Fis1 mitochondrial outer membrane targeting sequence for Cytb5mito (Cytb5mito-AC-GFP) also yields similar results. Scale bar: 5 µm. The table displays the degree of accumulation as quantified by the average coefficient of variance (standard deviation/mean pixel intensity in the mitochondrial region) ± the standard deviation for each probe compared to control probes. N indicates the number of cells analyzed. P-values determined by two-tailed Welch’s t-test. These results were reproducible across 7 independent experiments.