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Verification of CHK1 expression in HC, ND MM, and R/R MM samples. (A) mRNA expression of CHK1 in HC, NDMM and R/R MM samples were analyzed using qRT-PCR. The mRNA expression level of CHK1 was showed by relative expression level. (B) Western blot analysis of the protein expression of CHK1 in ND MM, and R/R MM samples. (C) Results of western blot analysis showing a drastic increase in R/R MM. P< 0.05 was considered statistically significant (*p<0.05, **P<0.01, ***P<0.001, ****P<0.0001).
