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. 2020 Nov 17;12(22):22495–22508. doi: 10.18632/aging.202221

Figure 4.

Figure 4

Distribution of ACE2 (green) in wt and HDAC1-depleted mES cells, non-differentiated and differentiated into cardiomyocytes. Immunofluorescence analyses were performed in (A) HDAC1 wt mESCs, (B) HDAC1 dn mESCs, (C) α-actinin (red)-positive cardiomyocytes generated from HDAC1 wt mESCs, and (D) α-actinin (red)-positive cardiomyocytes generated from HDAC1 dn mESCs. DAPI (blue) was used as a counterstain. (E) The nucleo/cytoplasmic ratio of the level of ACE2 is shown for HDAC1 wt mESCs. (F) The nucleo/cytoplasmic ratio of the level of ACE2 is shown for HDAC1 dn mESCs.