Table 1. Primers designed for qRT-PCR validation of candidate tsRNAs and mRNAs.

Gene name	Forward and reserve primer	Product length (bp)
rno-tRFi-Cys-20a	F: 5’ AGTCCGACGATCAGAGCATTT 3’	49
	R: 5’ GTGTGCTCTTCCGATCTGATCT 3’
rno-tRFi-Ser-25a	F: 5’ CCGACGATCATGGACTGCTA 3’	44
	R: 5’ CTTCCGATCTAGAGCACAATGG 3’
rno-tRFi-Gln-16a	F: 5’ GAGTTCTACAGTCCGACGATCT 3’	47
	R: 5’ CTTCCGATCTGGATTCAGAGT 3’
rno-tRF5-Glu-29a	F: 5’ ACAGTCCGACGATCTCCCATA 3’	57
	R: 5’ TGCTCTTCCGATCTAGGAATCC 3’
rno-tiR5-Lys-35b	F: 5’ ATCGCCCGGCTAGCTCAGT 3’	47
	R: 5’ TTCCGATCTAGAGTCCCATGCTC 3’
rno-tRFi-Leu-35a	F: 5’ GGATTTAGGCTCCAGTCATTTC 3’	49
	R: 5’ GTGCTCTTCCGATCTACCCA 3’
rno-tRF5-Ala-16a	F: 5’ GTTCAGAGTTCTACAGTCCGACG 3’	49
	R: 5’ CCGATCTACTGAGCTACATCCC 3’
U6	F: 5’ GCTTCGGCAGCACATATACTAAAAT 3’	89
	R: 5’ CGCTTCACGAATTTGCGTGTCAT 3’
Il1rn	F: 5’ GCCTGTCTTGTGTCAAGTCTGGAG 3’	150
	R: 5’ AGGCAAGTGATTCGAAGCTGGTG 3’
Mtr	F: 5’ CCAGCACAGAGCGTCCAAGATG 3’	161
	R: 5’ AGAGGAGCAACGAAGTCTGAGAGG 3’
Fzd1	F: 5’ TGAAGCATGACGGCACCAAGAC 3’	110
	R: 5’ GAAGTAGCAGGCGATGACGATGG 3’
Duox2	F: 5’ GCACAGCAGCCAGCATCTCC 3’	103
	R: 5’ GGAATGTAGCGGTTGAGGAAGGTC 3’
β-Actin	F: 5’ ACATCCGTAAAGACCTCTATGCC 3’	223
	R: 5’ TACTCCTGCTTGCTGATCCAC 3’