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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: Cell Rep. 2020 May 5;31(5):107610. doi: 10.1016/j.celrep.2020.107610

Figure 6. Disomes on Pro-Pro Motifs Bearing Positively Charged Nascent Chains Escape from Rescue by eIF5A.

Figure 6.

(A and B) The read distributions along human HLADPA1 (A) and STOML2 (B) mRNA from control (top) and eIF5A knockdown (bottom) samples are depicted, showing the A-site positions in the leading ribosome of the disome. The newly identified ribosome pause sites are highlighted in pink.

(C and E) Metagene analysis of disome reads around collision sites observed upon eIF5A knockdown (C) and found in naive cells (pause sites used in Figures 3 and 4; E).

(D) Amino acid sequences enriched in ribosome collision sites observed upon eIF5A knockdown.

(F) Net charge in the nascent chain around ribosome collision sites observed upon eIF5A knockdown and found in naive cells (pause sites used in Figures 3 and 4).

See also Figure S6 and Table S3.