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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: Cell Rep. 2020 May 5;31(5):107610. doi: 10.1016/j.celrep.2020.107610

Figure 7. Collided Ribosomes on XBP1u Are Rescued by the RQC Pathway.

Figure 7.

(A) The read distributions along human XBP1u mRNA are depicted, showing the A-site positions in the monosome (bottom) and in the leading ribosome of the disome (top). The known ribosome pause site (Yanagitani et al., 2011) is highlighted in pink.

(B) Western blot for the indicated proteins in naive or LTN1 knockout HEK293 cells.

(C) Western blot for proteins expressed from a reporter plasmid (left) in ZNF598 knockdown and control cells. Proteins with or without RNase treatment were separated by neutral PAGE to detect the peptidyl-tRNAs.

(D) Luciferase assay with the reporter with an XBP1u pause site sandwiched between self-cleavage 2A tags (left; same as Figure 1B) in ZNF598 knockdown and control cells. Data represent the mean and SD (n = 3).

See also Figure S7.